kumar, R and Andrabi, R and Tiwari, A and Praaksh, SS and Wig, N and Dutta, D and Sankhyan, A and Khan, L and Sinha, S and Luthra, K (2012) A novel strategy for efficient production of anti-V3 human scFvs against HIV-1 clade C. BMC METHODOLOGY.

Text 210_A novel strategy for efficient production of anti-V3 human scFvs against HIV-1 clade C.pdf Restricted to Registered users only Download (1016Kb)

Abstract

Production of human monoclonal antibodies that exhibit broadly neutralizing activity is needed for preventing HIV-1 infection, however only a few such antibodies have been generated till date. Isolation of antibodies by the hybridoma technology is a cumbersome process with fewer yields. Further, the loss of unstable or slowly growing clones which may have unique binding specificities often occurs during cloning and propagation and the strongly positive clones are often lost. This has been avoided by the process described in this paper, wherein, by combining the strategy of EBV transformation and recombinant DNA technology, we constructed human single chain variable fragments (scFvs) against the third variable region (V3) of the clade C HIV-1 envelope.

Item Type:	Article
Subjects:	Neurodegenerative Disorders Neuro-Oncological Disorders Neurocognitive Processes Neuronal Development and Regeneration Informatics and Imaging Genetics and Molecular Biology
Depositing User:	Dr. D.D. Lal
Date Deposited:	05 May 2017 09:54
Last Modified:	02 Jan 2018 09:33
URI:	http://nbrc.sciencecentral.in/id/eprint/92

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