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Kaushik, Deepak Kumar and Basu, Anirban (2013) Microglial activation: measurement of cytokines by flow cytometry. Methods Mol Biol, 1041. pp. 71-82. ISSN 978-1-62703-520-0

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Abstract

Cytokine measurement is a prerequisite to understand the inflammatory state of the body. Quantitative analysis of cytokines by Western blotting and ELISA is a daunting task as these are time-consuming and error-prone protocols. With the advent of flow cytometry, the estimation of cytokines using the classical antigen–antibody reaction has become a popular choice with researchers/clinicians. Here, we describe a protocol for multiple cytokine analysis using flow cytometry.

Item Type: Article
Subjects: Neurodegenerative Disorders
Neuro-Oncological Disorders
Neurocognitive Processes
Neuronal Development and Regeneration
Informatics and Imaging
Genetics and Molecular Biology
Depositing User: Dr. D.D. Lal
Date Deposited: 08 May 2017 09:41
Last Modified: 10 Dec 2021 07:25
URI: http://nbrc.sciencecentral.in/id/eprint/118

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