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Hervas, Ruben and Li, Liying and Majumdar, Amitabha and Fernandez Ramirez, Maria Del and Unruh, Jay R and Slaughter, Brian D and Galera Prat, Albert and Santana, Elena and Suzuki, Mari and Nagai, Yoshitaka and Bruix, Marta and Casas Tinto, Sergio and Menendez, Margarita and Laurents, Douglas V and Si, Kausik and Carrion Vazquez, Mariano (2016) Molecular Basis of Orb2 Amyloidogenesis and Blockade of Memory Consolidation. PLoS Biol, 14 (1). e1002361.


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Amyloids are ordered protein aggregates that are typically associated with neurodegenerative diseases and cognitive impairment. By contrast, the amyloid-like state of the neuronal RNA binding protein Orb2 in Drosophila was recently implicated in memory consolidation, but it remains unclear what features of this functional amyloid-like protein give rise to such diametrically opposed behaviour. Here, using an array of biophysical, cell biological and behavioural assays we have characterized the structural features of Orb2 from the monomer to the amyloid state. Surprisingly, we find that Orb2 shares many structural traits with pathological amyloids, including the intermediate toxic oligomeric species, which can be sequestered in vivo in hetero-oligomers by pathological amyloids. However, unlike pathological amyloids, Orb2 rapidly forms amyloids and its toxic intermediates are extremely transient, indicating that kinetic parameters differentiate this functional amyloid from pathological amyloids. We also observed that a well-known anti-amyloidogenic peptide interferes with long-term memory in Drosophila. These results provide structural insights into how the amyloid-like state of the Orb2 protein can stabilize memory and be nontoxic. They also provide insight into how amyloid-based diseases may affect memory processes

Item Type: Article
Subjects: Neurodegenerative Disorders
Neuro-Oncological Disorders
Neurocognitive Processes
Neuronal Development and Regeneration
Informatics and Imaging
Genetics and Molecular Biology
Depositing User: Dr. D.D. Lal
Date Deposited: 18 Feb 2020 05:12
Last Modified: 14 Dec 2021 05:04
URI: http://nbrc.sciencecentral.in/id/eprint/662

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