[feed] Atom [feed] RSS 1.0 [feed] RSS 2.0

Sharma, V and Dixit, D and Ghosh, S and Sen, Ellora (2011) COX-2 regulates the proliferation of glioma stem like cells. Neurochemistry International, 59. pp. 567-571.

[img] Text
COX-2 regulates the proliferation of glioma stem like cells.pdf
Restricted to Repository staff only

Download (464Kb) | Request a copy


Cancer stem-like cells (CSCs) possessing features of neural precursor cells (NPC) influence initiation, recurrence and chemoresistance of glioblastoma multiforme (GBM). As inflammation is crucial for glioblastoma progression we investigated the effect of chronic IL-1β treatment on CSCs derived from glioblastoma cell line U87MG. Exposure to IL-1β for 10 days increased (i) accumulation of 8-OHdG - a key biomarker of oxidative DNA damage; (ii) DNA damage response (DDR) indicators γH2AX, ATM and DNA-PK; (iii) nuclear and cytoplasmic p53 and COX-2 levels and (iv) interaction between COX-2 and p53. Despite upregulating p53 expression IL-1β had no effect on cell cycle progression, apoptosis or self renewal capacity of CSCs. COX-2 inhibitor Celecoxib reduced self renewal capacity and increased apoptosis of both control and IL-1β treated CSCs. Therefore the ability of COX-2 to regulate proliferation of CSCs irrespective of exposure to IL-1β, warrants further investigation of COX-2 as a potential anti-glioma target.

Item Type: Article
Subjects: Neurodegenerative Disorders
Neuro-Oncological Disorders
Neurocognitive Processes
Neuronal Development and Regeneration
Informatics and Imaging
Genetics and Molecular Biology
Depositing User: Dr. D.D. Lal
Date Deposited: 09 May 2018 03:37
Last Modified: 29 Nov 2021 09:09
URI: http://nbrc.sciencecentral.in/id/eprint/332

Actions (login required)

View Item View Item